A description of the microbe
How the microbe is transmitted to humans.
Who is most likely to be infected by the microbe and the circumstances under which they may be infected.
How to avoid transmission of the microbe.
What the signs and symptoms are for the infection.
The treatment and any side-effects
How do you think the existence of multidomain proteins relates to complexity in higher eukaryotes?
The attached paper is for your reading, if interested. It discussed about structure-based inhibitor.
How can you define domain and motif? Is there any significance in bioinformatics or in proteomics?
Why Snare complex is important in normal muscle movement?
If your role were to generate an inactive mutant of the protein encoded by the gene sequence below in an E. coli expression system, show the primers you would design to generate that mutant. Base your design on the mutations tested in adenylate kinase reported in Saraste et. al, (1990) (4 marks).
List the important features of the primer that you have checked for to ensure the success of your experiment (2 marks).
Once you have designed your primers, draw out a flow diagram, with a few explanatory notes, showing the experimental steps needed to perform this mutagenesis experiment (refer to the lecture notes for help) (2marks).
Isolation of chromosomal DNA from E-coli:Discuss?
Gene editing methods have been around for years but involved cumbersome mechanisms. In 2015 the CRISPR gene editing system has made this method easy to do. One Chinese researcher has modified human embryos so that they are not susceptible to HIV. His work was condemned by the international scientific society:Discuss
Answers should be double spaced; 11-12 font size; standard margins. Proper grammar and paragraph format is expected. All answers should be written in complete sentences.
A recent clinical study demonstrated a possible connection between coffee consumption and heart health. The researchers performed a 6-week trial with 191 individuals, all of whom were heavy coffee drinkers (average of 5 cups/day) before the experiment, but none of whom were smokers. They divided the 191 participants into three groups: no coffee consumption, 1-3 cups/day, and four or more cups/day. They measured blood levels of cholesterol and homocysteine as indicators of heart health. Only the no-coffee group showed a significant drop in both indicators. Previous studies had demonstrated that decreases of the magnitude observed in the no-coffee group could cut the incidence of heart disease by 10-15%.
What is the problem/question being studied?
What is the hypothesis?
What is the experimental variable?
d) List the controls.
e) What is being measured to test the hypothesis?
Several factors (including agarose gel concentration, time and current) affect migration of DNA fragments through the agarose gel. Briefly explain how each of these factors affects DNA migration. [3 marks]
Do DNA fragments move towards the positive or negative end of the gel box? Explain your answer. [2 marks]
What is the purpose of the Tris-Acetate-EDTA (TAE) buffer that the agarose gel is prepared with and submerged in for running? What would happen if you used water to prepare and run the gel instead of TAE buffer? (You should conduct an internet search to answer this question) [2 marks]
If the student is homozygous for the brown allele, how many bands will they see in the lanes for the blue and brown allele samples? (circle one) [1 mark]
If the student is homozygous for the blue allele, how many bands will they see in the lanes for the blue and brown allele samples? (circle one) [1 mark]
If the student is heterozygous, how many bands will they see in the lanes for the blue and brown allele samples? (circle one) [1 mark
Define and give examples (other than those discussed in class) of competition. Can use internet for examples.
Give the scientific name of the plant, in italics and with the first letter of the genus capitalized as shown in class:
Scientific name: Brassica rapa
Family: Brassicaceae
Common name: Rossette Standard
State your hypothesis, which should include all 2 plant population density treatments.
State whether you accept or reject the hypothesis (es)
If you accept your hypotheses, EXPLAIN WHY you got the results that you did
If you reject your hypotheses, EXPLAIN what you expected and WHY, and explain why your results turned out differently.
Why does hair fall out?
How does a fracture heal?
Why do we get muscle cramps?
How does my body react during “flight or fight” response?
How do we detect and interpret music?
Why do we get dizzy?•
Why and when should you prefer PSI-BLAST over blastp?
Why e-values differ in PSI-BLAST from first search to second iteration?
What does PSSM stand for? Which BLAST search program uses the PSSM?
In what situation one needs to verify two putative homologs using pairwise alignment and why?
Use PSI-BLAST to find homologues of protein P14629 (select uniprot/swissprot database),
limited to vertebrates.
What threshold did you use and why?
What type of enzyme shows up in almost every description?
Find the protein with accession number Q9UQ84.2. What is the enzyme?
What is the E value for Q9UQ84.2? Confirm homology between Q9UQ84.2 and P14629 using pairwise BLAST alignment.
What is the E value?Are they homologues based on that E value?
Why might BLAST have not found all homologs on the first iteration?
Other than pairwise alignment, describe what else you could have done on your computer with BLAST and alignment tools to try to confirm homology between Q9UQ84.2 and P14629.
Describe what else you could do in the wet lab to try to confirm homology between Q9QUQ84.2 and P14629.
