Lab report 8 Extraction of DNA from Wheat Germ
In the space provide below, draw a four-nucleotide piece (e.g. ATGC) of DNA, drawing out the FULL structure showing the four different nitrogenous bases that occur in DNA and showing it in its ionization state at physiological pH. Draw the four bases (A, C, T, G) and their full structure so the backbone of the DNA and the nitrogenous bases.
Describe the difference between the genomic DNA you isolated in this experiment and “plasmid” DNA used in recombinant DNA technology.
1.6 to 2.0 Ratio = Pure DNA
What is the A260 value for your DNA sample? ____0.334___________ .
What is the A280 value for your DNA sample? ____0.325___________ .
What is the A260/A280 ratio for your DNA sample?
Based upon the A260/A280 ratio of your sample, what can you say about the purity of your isolated DNA?
What was the weight of the DNA you isolated?
You started with 2g of raw wheat germ. Using this knowledge calculate the apparent % of your raw wheat germ that is supposedly DNA. Show your work below.
Apparent % DNA in wheat germ: ____________.
Here is a rule of thumb used to calculate the approximate DNA concentration in a solution, based upon the A260 value: A DNA solution of concentration 50 µg/ml gives an A260 value of 1.
Calculate the total amount of DNA in your isolated wheat germ DNA sample based upon your measured A260. Take into account any dilutions you may have made. Show your calculations below.
Total amount of DNA in wheat germ (based on A260): ____________.
% DNA in wheat germ (based on A260): ____________.
Which method of measuring the amount of DNA in your wheat germ gives the larger amount of DNA?
Which method do you think is a more accurate way of measuring the amount of DNA in a sample?Why?
