Explain the relevance of testing the phenotype of RNAi treatment for 400 genes on wild type or MIT mutant c. elegans (make sure to address why MIT mut worms were used and what research group(s) discovered this particular MIT mut worm phenotype).

Hypoxia – TAF-4 is required for the life extension of isp-1, clk-1 and tpk-1 Mit mutants

1.Explain the relevance of testing the phenotype of RNAi treatment for 400 genes on wild type or MIT mutant c. elegans (make sure to address why MIT mut worms were used and what research group(s) discovered this particular MIT mut worm phenotype).

2. What important discovery does this paper make about TAF-4 and HIF-1?

3. In worms, approximately how many genes does HIF-1 regulate during hypoxia?

4.  What happens to the longevity of worms when RNAi is used to knockdown HIF-1 in MIT mutant worms?