Biology assignment primer design
If your role were to generate an inactive mutant of the protein encoded by the gene sequence below in an E. coli expression system, show the primers you would design to generate that mutant. Base your design on the mutations tested in adenylate kinase reported in Saraste et. al, (1990) (4 marks).
List the important features of the primer that you have checked for to ensure the success of your experiment (2 marks).
Once you have designed your primers, draw out a flow diagram, with a few explanatory notes, showing the experimental steps needed to perform this mutagenesis experiment (refer to the lecture notes for help) (2marks).
