Chemistry pre lab questions / Enzyme-Catalyzed Oxidation of Tyrosine
Briefly describe the experimental procedure. Explain how the wavelength is chosen and why.RESULTS AND DISCUSSION
1.Summarize your data (concentrations, rates and other relevant parameters) in a table
2.Consider the glassware used for the solutions in order to calculate the uncertainty in the concentration (no uncertainty is provided for the stock solution in this case).
3.Plot the 6 kinetic curves. Comment on the shape of the curves.
4.Use the data in the output table for further calculations / graphing. Consider if your data was measured at 310 nm or 485 nm. What are the implications?
5.Calculate the standard deviation from the repeated runs (runs 1-3). What conclusions can you make in terms of minimizing the error in the measurement –how would you ideally construct the experiment?
6.Generate the Lineweaver-Burk Plot and extend the trendline, so that it clearly shows the x-intercept. Use the LINEST function to extract the values of Kmand Vmax from your graph. Make sure to report the uncertainties in their values as well. Calculatek3.
7.Test the mechanism proposed using equation (19) by calculating the theoretical values of V using Kmand Vmax from your graph and [S] from your experiment.
8.Tabulate this data and compare it to the values for V you obtained experimentally. Note that enzyme activity is variable which, in turn, affects reproducibility. Therefore, you will be marked based upon your analysis of the data and not on the results obtained.
9.Discuss the value of Vmax and Km and compare with similar studies in the literature