Practical report
Explain the relation between genomic DNA and RNA, describe what an intron is. What is the purpose of the experiment? Shortly summarise the outcome of the sequence analysis of rad21, do you think it has an intron? Explain the experimental design and how it should allow you to detect the absence or presence of the predicted intron.
What is the function of each of the main steps in the protocol? What is the expected result in case of the intron being present? What if the intron is absent? Methods (10 out of 100 Marks; Max 100 words)
Here you can refer to the protocol used in the practical. Describe any deviations (if any) from the protocol.
Results (25 out of 100 Marks; Max 500 words, excluding tables and figure legends) For each of the main steps, present any results you may have obtained. How much RNA did you purify?
How much genomic DNA? Present your calculations. Present the picture of the gel and label it properly: each lane should be labelled, mark the sizes of the DNA ladder. Provide a figure legend.
Determine the sizes of the bands that you can see and mention these in the text. Most importantly: describe and interpret your results in the main text, talk the reader through what you conclude from your data and why.
Discussion (25 out of 100 Marks; Max 500 Words)
