Lab 5
Several factors (including agarose gel concentration, time and current) affect migration of DNA fragments through the agarose gel. Briefly explain how each of these factors affects DNA migration. [3 marks]
Do DNA fragments move towards the positive or negative end of the gel box? Explain your answer. [2 marks]
What is the purpose of the Tris-Acetate-EDTA (TAE) buffer that the agarose gel is prepared with and submerged in for running? What would happen if you used water to prepare and run the gel instead of TAE buffer? (You should conduct an internet search to answer this question) [2 marks]
If the student is homozygous for the brown allele, how many bands will they see in the lanes for the blue and brown allele samples? (circle one) [1 mark]
If the student is homozygous for the blue allele, how many bands will they see in the lanes for the blue and brown allele samples? (circle one) [1 mark]
If the student is heterozygous, how many bands will they see in the lanes for the blue and brown allele samples? (circle one) [1 mark
